The Non-toxicity of Bacillus anthracis Cell Material

Reverting and Non-reverting Rough Variants of Bacillus Anthracis.

Ruling Out Bacillus anthracis

Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround times, and a lighter workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse blood agar, motility testing after isolates had a 2-h incubation in trypticase soy broth, and screening isolates with a B. anth...

Lecithinase from Bacillus anthracis.

When Bacillus anthracis is grown in egg yolk medium, a reaction occurs which is presumably due to lecithinase (Colmer, 1948; McGaughey and Chu, 1948; Chu, 1949). In liquid egg yolk medium the lecithinase reaction is characterized by the formation of an opalescent suspension and flocculation of particles which rise to the top of the medium as a curd. A test such as this is usually referred to as...

Pyrosequencing Bacillus anthracis

Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single n...

Immunological analysis of cell-associated antigens of Bacillus anthracis.

Sera from Hartley guinea pigs vaccinated with a veterinary live spore anthrax vaccine were compared with sera from guinea pigs vaccinated with the human anthrax vaccine, which consists of aluminum hydroxide-adsorbed culture proteins of Bacillus anthracis V770-NP-1R. Sera from animals vaccinated with the spore vaccine recognized two major B. anthracis vegetative cell-associated proteins that wer...